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Saturday, March 20, 2010

Medicinal Chemistry Part 2 - Answers to Questions from Soon-To-Be-Graduates

At January's CSU Biotechnology Symposium, soon-to-be graduates from life sciences programs had many questions about relevant career paths.  This is the second of three posts addressing questions specifically related to medicinal chemistry.  The questions addressed in this post are:
  • How does molecular modeling influence medicinal chemistry?
  • How does natural product research relate to medicinal chemistry?
  • How can natural products be matched to enzyme activity?
How does molecular modeling influence medicinal chemistry?

In my last post, the question "What is medicinal chemistry?" was answered. In that post, I discussed the role of a medicinal chemist in the context of a drug discovery program.  In such a program, success is dependent upon the synergistic activities of scientists of many disciplines.  Some of these disciplines are:
  • organic chemistry
  • biochemistry
  • biology
  • pharmacology
  • analytical chemistry
  • computational chemistry
Organic chemistry is necessary for the generation of drug candidates. Biochemistry is used to design the enzyme-based assays used to test drug candidates.  Likewise, biology is used to design the cell-based assays and animal models of diseases.  Pharmacology is applied to the actual testing of drug candidates in live animals and analytical chemistry is necessary to provide meaningful data regarding blood serum concentrations as well as initial drug purity assessments.  Based on these specific roles, it is entirely appropriate to wonder where computational chemistry fits in.

Computational chemistry utilizes mathmatical algorithms and computer technology to: 
  • predict the achievable structural conformations of drug candidates
  • image protein/enzyme structures based on X-ray crystallographic data
  • predict protein/enzyme structures based on X-ray crystallographic data of related proteins/enzymes
  • compare biological data of related structures as a tool to predict the activity of planned compounds
  • study the enzyme binding site in the presence and/or absence of drug candidates
  • design novel structures optimized to the shape and properties of relevant enzyme binding sites.
Because all of the above applications of computation chemistry effectively visualize what cannot be directly seen, each of these systems is a model designed to represent intended interactions within biological systems. Because these systems are models, computational chemistry is frequently termed "molecular modeling."  While this technology was routinely unavailable to medicinal chemists until the 1980s, such technology is now indispensable in guiding the activities of medicinal chemists to more rapid successes. 

How does natural product research relate to medicinal chemistry?

Nature has provided numerous biologically active compounds useful for medicinal purposes.  Such compound classes include opiods, antibiotics and antifungals.  While some compounds from these classes are commonly used as medicines (morphine, cocaine, penicillin, erythromycin, taxol, nystatin), most induce undesirable and potentially toxic side effects when administered to humans.  The reason for this is clear - these compounds were designed by nature to benefit their non-human host organisms.

Because of nature's relatively inefficient optimization of biologically active molecules for human use, most natural products are best utilized as lead molecules in drug discovery programs.  Through the systematic modification of natural substances, drug-like properties can be enhanced while minimizing undesirable and/or off-target effects.  Thus, natural product research provides medicinal chemists a rich and valuable goldmine of lead molecules and potential drug candidates.

How can natural products be matched to enzyme activity?

Natural products are organic molecules produced by living organisms. While there are numerous living organisms that produce interesting substance, plants fungi and marine creatures generally receive the most attention.

The process of natural product discovery generally involves homogenization of large quantities of a given species such as a marine sponge.  The liquefied sponge is then extracted with organic solvents. The materials being dissolved into the organic solvents are then isolated and separated by techniques such as high pressure liquid chromatography (HPLC).  This initial chromatography step produces fractions that contain multiple natural compounds.  As only natural products with biological activity are generally of interest, these crude fractions are screened against various enzymes.  If a given fraction shows interesting activity, it is further purified to isolate its individual molecular components.  These components are then screened against the enzymes of interest and the most active component is identified.

From this point, a map from a given sponge to a given natural product is identified.  Using this map, the natural product of interest can be repeatedly isolated from the same species.  However, the structure of the natural product is not yet known.  In order to establish the structure, larger quantities must be isolated and subjected to analytical techniques such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy.  Additional structural information can be obtained by studying the products of chemical degradation.  When a final structure is proposed, often the best proof of structure is total synthesis - the process of preparing a natural product from non-natural sources using synthetic organic chemistry. In fact, it is this process of total synthesis that provides some of the best training for graduate students interested in entering industries dependent on the discovery of pharmaceuticals, polymers and agrochemicals.

Sunday, March 7, 2010

Medicinal Chemistry Part 1 - Answers to Questions from Soon-To-Be-Graduates

Students preparing to enter the workforce have a great many questions. Some of these relate generally to career paths while others relate more specifically to various professions.  In my last two posts, I focused on career paths.  I hope the answers I provided are helpful and look forward to reading your comments.

At January's CSU Biotechnology Symposium, I had the opportunity to listen to both questions and concerns of soon-to-be graduates from life science programs.  Those participating at my table (medicinal chemistry) provided me with a forum to address qustions specific to my area of expertise.  These questions, related to medicinal chemistry, are:  
  • What is medicinal chemistry?
  • Do medicinal chemists participate in the testing of compounds?
  • What is a typical day like for a medicinal chemist?
  • How does molecular modeling influence medicinal chemistry?
  • How does natural product research relate to medicinal chemistry?
  • How can natural products be matched to enzyme activity?
  • What is the impact of small protein design?
  • What are the advantages/disadvantages of biologics vs small molecule drugs?
In this and the following posts, I will address all of these questions.

What is medicinal chemistry?

Medicinal chemistry is the discipline focused on the discovery of new medications.

Many medications are derived from natural sources such as plants, sponges and animal toxins/venoms.  Specific examples include aspirin, morphine and cocaine.  However, while nature provides many biologically important molecules, these molecules are the result of evolutionary pressure upon the organisms from which they are isolated.  As such, they are not necessarily optimal for use in humans. In order to make use of such naturally occurring compounds, medicinal chemists work to modify these structures in order to enhance desirable properties and minimize toxic side effects.

Complementary to naturally occurring compounds are the vast libraries of chemicals available for biological screening.  These compounds are generally man-made and provide equally enticing structures as lead molecules for drug discovery programs.  

Because medicinal chemistry involves the systematic modification of natural or man-made organic molecules, a background in organic chemistry is essential to the medicinal chemist.

Do medicinal chemists participate in the testing of compounds?

Medicinal chemistry is only one component essential to the drug discovery process.  Another aspect of this process involves the testing of the compounds that are produced by medicinal chemists.  The biological assays generally include enzyme assays, cell-based assays and biologically relevant animal models of diseases.  These studies are generally designed and carried out by biologists and pharmacologists.  

Medicinal chemists do not carry out biological assays.  However, the feedback generated by biologists and pharmacologists is essential to helping medicinal chemists design their next modifications to lead molecules.  Thus by correlating the structures of related compounds to their respective biological data, medicinal chemists can determine if they are moving towards or away from improved drug candidates.

Although medicinal chemists do not generally participate in compound screening, it is not unheard of.  Early in my career, I was curious as to whether a class of matrix metalloproteinase inhibitors (MMPI)s would act as inhibitors of endothelin converting enzyme (ECE).  At the time, there was no enzyme assay available for ECE.  However, there was an way to measure ECE activity in rats by measuring their blood pressure in response to dosages of big endothelin (big-ET) in the presence or absence of an MMPI.  With this knowledge, a colleague of mine in the pharmacology department taught me how to surgically cannulate rat carotid arteries and, together, we were able to demonstrate that MMPIs could inhibit ECE. This was the only time in my career that I actually participated in biological assays and it taught me a great deal about the role and importance of pharmacologists to the drug discovery process.

What is a typical day like for a medicinal chemist?

Medicinal chemists typically spend their days making target molecules for biological testing.  Approximately 70% of their time is spent initiating reactions, completing reactions and isolating/purifying products.  The remaining 30% may be spent generating analytical data (NMR, HPLC, MS) for isolated compounds, reviewing biological data provided by biologists, searching scientific literature for information/procedures needed for chemical transformations, and attending group meetings.

The role of a medicinal chemist is highly intertwined with related operational groups such as biology, pharmacology, formulations and process chemistry.  The medicinal chemist must be able to effectively communicate with all of these disciplines in order to share necessary information and glean important insight valuable to program advancement.  Each day is different from the last and each day begins with the hope of new discoveries.